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p ampk  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p ampk
    P Ampk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 779 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+ampk/Phospho-AMPKalpha+(Thr172)+Rabbit+mAb/pm42887617-67-75-78
    Average 96 stars, based on 779 article reviews
    p ampk - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    other:

    Article Title: A promising magnesium-related alloy with metabolic reprogramming and antitumor effects in hepatocellular and pancreatic cancer
    Article Snippet: Equal amounts of protein were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, LG18-1501M − 1H).

    Article Title: Copper Depletion Nanoparticles Potentiate Cancer Immunotherapy by Avoiding Innate and Adaptive Immune Resistance.
    Article Snippet: The antibodies and reagents used in this research were as follows: β-Actin (#AF7018, Affinity), PD-L1 (#DF6526, Affinity; #ab205921, Abcam), CD47 (#DF6649, Affinity; #ab218810, Abcam), p-AMPK (#2535, Cell Signaling Technology (CST)), AMPK (#2532, CST), Ki67 (#AF0198, Affinity), IFN- γ (#DF6045, Affinity; #ab231036, Abcam), Granzyme B/H (#AF0175, Affinity; #ab134933, Abcam), CD3 (78588S, CST; #ab16669, Abcam), CD4 (ab288724, Abcam; #ab133616, Abcam), CD8 (98941S, CST; #ab237709, Abcam), C-MYC (#13987, CST), Bcl-2 (#15071, CST), Cleaved-Caspase3 (#AF7022, Affinity), N-cadherin (#AF5239, Affinity), SLC31A1 (#ab133385, Abcam), Goat Anti-Rabbit IgG HRP (#S0001, Affinity), CuCl2 ⋅2H2 O (C805298, Macklin), Metformin (HY-B0627, MCE), Acadesine (AICAR) (HY-13417, MCE), BC1618 (HY-134656, MCE), Cycloheximide (CHX) (C729197, aladdin), Ammonium tetrathiomolybdate (ATTM) (A828261, Macklin), Bis(thiobenzoyl) disulfide (DTBS) (B909295, Macklin), Tris(2-pyridylmethyl)amine (CDA).

    Blocking Assay:

    Article Title: Exploring the therapeutic mechanism of kaempferol in acute lung injury: Network pharmacology and experimental validation.
    Article Snippet: Acute lung injury (ALI) is a common and life-threatening respiratory disorder characterised by severe inflammation and persistently high mortality rates.. Kaempferol (KAE), a natural flavonoid compound, has diverse biological activities and holds potential for therapeutic applications.. In this study, we employed a network pharmacology approach to predict the mechanism through which KAE mitigates ALI; this mechanism was subsequently validated experimentally in a mouse model of ALI.

    Membrane:

    Article Title: Exploring the therapeutic mechanism of kaempferol in acute lung injury: Network pharmacology and experimental validation.
    Article Snippet: Acute lung injury (ALI) is a common and life-threatening respiratory disorder characterised by severe inflammation and persistently high mortality rates.. Kaempferol (KAE), a natural flavonoid compound, has diverse biological activities and holds potential for therapeutic applications.. In this study, we employed a network pharmacology approach to predict the mechanism through which KAE mitigates ALI; this mechanism was subsequently validated experimentally in a mouse model of ALI.

    Western Blot:

    Article Title: Low-dose juglone attenuates pressure overload-induced cardiac hypertrophy through Lys207-dependent FoxO1 activation and mitochondrial protection.
    Article Snippet: Cell Counting Kit-8 (CCK-8-100T, 96992) was purchased from Sigma-Aldrich. .. All primary antibodies against the indicated proteins used for immunoblotting, immunoprecipitation and immunostaining analysis in the present study are listed as follows: α-SMA (A2547, Sigma-Aldrich, USA), Collagen III (Abcam, ab184993), BNP (Abcam, ab19645), MYH7 (Cat: 22280-1, Protein tech), FoxO1 (#2880, CST), Pan-acetylation (Abcam, ab190479), Flag (F9291, SigmaAldrich, USA), α-actinin (Abcam, ab68194), CTnT (Abcam, ab209813), GAPDH (Abcam, ab8245), H3(Abcam, ab1791), β-actin (#8235, CST), Sesn3 (Abcam, ab97792), Pin1 (Abcam, 192036), 4-HNE (Thermo Fisher, MA5–27570), Sirt1 (Abcam, ab110304), P-AMPK (Thr172, #50081, CST), AMPK (#2532, CST). .. Biotin antibody (Abcam, ab53494) and FoxO1 antibody (#14952, CST) were used for assessing the colocalization by immunofluorescence in NMCMs.

    Immunoprecipitation:

    Article Title: Low-dose juglone attenuates pressure overload-induced cardiac hypertrophy through Lys207-dependent FoxO1 activation and mitochondrial protection.
    Article Snippet: Cell Counting Kit-8 (CCK-8-100T, 96992) was purchased from Sigma-Aldrich. .. All primary antibodies against the indicated proteins used for immunoblotting, immunoprecipitation and immunostaining analysis in the present study are listed as follows: α-SMA (A2547, Sigma-Aldrich, USA), Collagen III (Abcam, ab184993), BNP (Abcam, ab19645), MYH7 (Cat: 22280-1, Protein tech), FoxO1 (#2880, CST), Pan-acetylation (Abcam, ab190479), Flag (F9291, SigmaAldrich, USA), α-actinin (Abcam, ab68194), CTnT (Abcam, ab209813), GAPDH (Abcam, ab8245), H3(Abcam, ab1791), β-actin (#8235, CST), Sesn3 (Abcam, ab97792), Pin1 (Abcam, 192036), 4-HNE (Thermo Fisher, MA5–27570), Sirt1 (Abcam, ab110304), P-AMPK (Thr172, #50081, CST), AMPK (#2532, CST). .. Biotin antibody (Abcam, ab53494) and FoxO1 antibody (#14952, CST) were used for assessing the colocalization by immunofluorescence in NMCMs.

    Immunostaining:

    Article Title: Low-dose juglone attenuates pressure overload-induced cardiac hypertrophy through Lys207-dependent FoxO1 activation and mitochondrial protection.
    Article Snippet: Cell Counting Kit-8 (CCK-8-100T, 96992) was purchased from Sigma-Aldrich. .. All primary antibodies against the indicated proteins used for immunoblotting, immunoprecipitation and immunostaining analysis in the present study are listed as follows: α-SMA (A2547, Sigma-Aldrich, USA), Collagen III (Abcam, ab184993), BNP (Abcam, ab19645), MYH7 (Cat: 22280-1, Protein tech), FoxO1 (#2880, CST), Pan-acetylation (Abcam, ab190479), Flag (F9291, SigmaAldrich, USA), α-actinin (Abcam, ab68194), CTnT (Abcam, ab209813), GAPDH (Abcam, ab8245), H3(Abcam, ab1791), β-actin (#8235, CST), Sesn3 (Abcam, ab97792), Pin1 (Abcam, 192036), 4-HNE (Thermo Fisher, MA5–27570), Sirt1 (Abcam, ab110304), P-AMPK (Thr172, #50081, CST), AMPK (#2532, CST). .. Biotin antibody (Abcam, ab53494) and FoxO1 antibody (#14952, CST) were used for assessing the colocalization by immunofluorescence in NMCMs.

    Analysis:

    Article Title: Low-dose juglone attenuates pressure overload-induced cardiac hypertrophy through Lys207-dependent FoxO1 activation and mitochondrial protection.
    Article Snippet: Cell Counting Kit-8 (CCK-8-100T, 96992) was purchased from Sigma-Aldrich. .. All primary antibodies against the indicated proteins used for immunoblotting, immunoprecipitation and immunostaining analysis in the present study are listed as follows: α-SMA (A2547, Sigma-Aldrich, USA), Collagen III (Abcam, ab184993), BNP (Abcam, ab19645), MYH7 (Cat: 22280-1, Protein tech), FoxO1 (#2880, CST), Pan-acetylation (Abcam, ab190479), Flag (F9291, SigmaAldrich, USA), α-actinin (Abcam, ab68194), CTnT (Abcam, ab209813), GAPDH (Abcam, ab8245), H3(Abcam, ab1791), β-actin (#8235, CST), Sesn3 (Abcam, ab97792), Pin1 (Abcam, 192036), 4-HNE (Thermo Fisher, MA5–27570), Sirt1 (Abcam, ab110304), P-AMPK (Thr172, #50081, CST), AMPK (#2532, CST). .. Biotin antibody (Abcam, ab53494) and FoxO1 antibody (#14952, CST) were used for assessing the colocalization by immunofluorescence in NMCMs.

    Incubation:

    Article Title: Curcumin protects retinal photoreceptors in Glaucoma by activating prostaglandin D2 synthase-dependent NRF2 signaling.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.



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    RNA-Seq analysis and validation of mouse liver following FSP intervention. (A) Heatmap of hepatic gene expression profiles in vehicle group and FSP-2X group, with red and green indicating up- and down-regulated genes respectively ( n = 3); (B) Volcano plot displaying overall distribution of DEGs in FSP-treated liver tissues ( n = 3); (C) Bubble chart of KEGG pathway enrichment analysis for DEGs ( n = 3); (D) Heatmap visualization of differentially expressed genes ( n = 3); (E) mRNA expression analysis of target genes ( n = 6); (F, G) Expression levels and semi-quantitative analysis of key proteins in <t>AdipoRs-AMPK</t> signaling pathway ( n = 3); (H, I) Regulatory effects of FSP on proteins related to inflammation, oxidative stress, mitochondrial function and lactate metabolism ( n = 3); (J) mRNA levels of mitochondrial biogenesis genes Nrf1 (NRF1) and Tfam (TFAM) ( n = 6); (K) Mitochondrial DNA copy number in mouse liver tissues ( n = 6); (L–Q) Immunohistochemical staining and statistical analysis of PGC1α, TFAM and COXIV proteins (Scale bar: 20 μm, Total magnification: 200x, n = 5); (R) Hepatic ATP content ( n = 5); (S) ROS levels in liver tissues ( n = 4). Results are expressed as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to the vehicle group.
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    Alteration of muscle FGF21 and downstream signaling in Ogg1 Tg mice. A , volcano plot of differentially expressed genes (DEGs) in skeletal muscle of Ogg1 Tg mice. Fgf21 is the most upregulated gene among all DEGs. B , Fgf21 gene expression was measured in major tissues of WT and Ogg1 Tg mice using quantitative PCR. Gene expressions were normalized to 18S rRNA expression. C , plasma FGF21was measured in WT and Ogg1 Tg mice using ELISA. D and E , phosphorylation of <t>AMPK</t> and ACC was measured in the skeletal muscle of WT and Ogg1 Tg mice using PAGE, and images were quantified using ImageJ. (n = 3–7); data are expressed as mean ± SD; ∗ p < 0.05 relative to WT. ACC, acetyl-CoA carboxylase; AMPK, AMP-activated protein; FGF21, fibroblast growth factor 21; Ogg1 Tg , OGG1-transgenic.
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    Image Search Results


    RNA-Seq analysis and validation of mouse liver following FSP intervention. (A) Heatmap of hepatic gene expression profiles in vehicle group and FSP-2X group, with red and green indicating up- and down-regulated genes respectively ( n = 3); (B) Volcano plot displaying overall distribution of DEGs in FSP-treated liver tissues ( n = 3); (C) Bubble chart of KEGG pathway enrichment analysis for DEGs ( n = 3); (D) Heatmap visualization of differentially expressed genes ( n = 3); (E) mRNA expression analysis of target genes ( n = 6); (F, G) Expression levels and semi-quantitative analysis of key proteins in AdipoRs-AMPK signaling pathway ( n = 3); (H, I) Regulatory effects of FSP on proteins related to inflammation, oxidative stress, mitochondrial function and lactate metabolism ( n = 3); (J) mRNA levels of mitochondrial biogenesis genes Nrf1 (NRF1) and Tfam (TFAM) ( n = 6); (K) Mitochondrial DNA copy number in mouse liver tissues ( n = 6); (L–Q) Immunohistochemical staining and statistical analysis of PGC1α, TFAM and COXIV proteins (Scale bar: 20 μm, Total magnification: 200x, n = 5); (R) Hepatic ATP content ( n = 5); (S) ROS levels in liver tissues ( n = 4). Results are expressed as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to the vehicle group.

    Journal: Frontiers in Nutrition

    Article Title: The herbal pair of Smilax glabra Roxb. and Ficus hirta Vahl. improves exercise performance by regulating mitochondrial function via the adiponection receptors-mediated AMPK signaling pathway

    doi: 10.3389/fnut.2026.1782001

    Figure Lengend Snippet: RNA-Seq analysis and validation of mouse liver following FSP intervention. (A) Heatmap of hepatic gene expression profiles in vehicle group and FSP-2X group, with red and green indicating up- and down-regulated genes respectively ( n = 3); (B) Volcano plot displaying overall distribution of DEGs in FSP-treated liver tissues ( n = 3); (C) Bubble chart of KEGG pathway enrichment analysis for DEGs ( n = 3); (D) Heatmap visualization of differentially expressed genes ( n = 3); (E) mRNA expression analysis of target genes ( n = 6); (F, G) Expression levels and semi-quantitative analysis of key proteins in AdipoRs-AMPK signaling pathway ( n = 3); (H, I) Regulatory effects of FSP on proteins related to inflammation, oxidative stress, mitochondrial function and lactate metabolism ( n = 3); (J) mRNA levels of mitochondrial biogenesis genes Nrf1 (NRF1) and Tfam (TFAM) ( n = 6); (K) Mitochondrial DNA copy number in mouse liver tissues ( n = 6); (L–Q) Immunohistochemical staining and statistical analysis of PGC1α, TFAM and COXIV proteins (Scale bar: 20 μm, Total magnification: 200x, n = 5); (R) Hepatic ATP content ( n = 5); (S) ROS levels in liver tissues ( n = 4). Results are expressed as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to the vehicle group.

    Article Snippet: The specific antibodies used were as follows: AdipoR1 (Abclonal, A1509), AdipoR2 (Abclonal, A12777), p-AMPK (Abclonal, AP1441), AMPK (Abclonal, A1229), p-LKB1 (Abclonal, AP0601), LKB1 (Abclonal, A22636), PGC1α (Proteintech, 66369-1-Ig), SIRT1 (Proteintech, 13161-1-AP), LDHB (Proteintech, 14824-1-AP), COXIV (Cell Signaling Technology, 3E11), TFAM (Abclonal, A3173), NRF2 (Proteintech, 16396-1-AP), HO-1 (Abclonal, A19062), TNFα (Proteintech, 80258-6-RR), COX2 (Abclonal, A3560), and β-actin (Proteintech, 20536-1-AP).

    Techniques: RNA Sequencing, Biomarker Discovery, Gene Expression, Expressing, Immunohistochemical staining, Staining

    Effects of FSP serum treatment on gene expression in HepG2 cells. (A) Experimental design of FSP serum treatment in HepG2 cells (Created in https://BioRender.com ); (B, C) Impact of FSP serum on HepG2 cell proliferation capacity and quantitative analysis ( n = 6); (D) Transcript levels of differentially expressed genes and mitochondrial biogenesis-related genes (Nrf1/NRF1, Tfam/TFAM) in HepG2 cells ( n = 6); (E, F) Protein expression and semi-quantification of key AdipoRs-AMPK pathway components in HepG2 cells ( n = 3); (G, H) Effects of FSP serum on inflammation, oxidative stress, mitochondrial function and lactate metabolism-related proteins with semi-quantitative analysis ( n = 3); (I) Immunofluorescence co-localization of SIRT1 and PGC-1α proteins in HepG2 cells (Scale bar: 20 μm, Total magnification: 200x); (J, K) Immunofluorescence detection of mitochondrial function proteins COXIV and TFAM (Scale bar: 20 μm, Total magnification: 200x); (L, M) Mitochondrial membrane potential assay (JC-1) and analysis (Scale bar: 20 μm, Total magnification: 200x, n = 5). Results are expressed as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to the vehicle group.

    Journal: Frontiers in Nutrition

    Article Title: The herbal pair of Smilax glabra Roxb. and Ficus hirta Vahl. improves exercise performance by regulating mitochondrial function via the adiponection receptors-mediated AMPK signaling pathway

    doi: 10.3389/fnut.2026.1782001

    Figure Lengend Snippet: Effects of FSP serum treatment on gene expression in HepG2 cells. (A) Experimental design of FSP serum treatment in HepG2 cells (Created in https://BioRender.com ); (B, C) Impact of FSP serum on HepG2 cell proliferation capacity and quantitative analysis ( n = 6); (D) Transcript levels of differentially expressed genes and mitochondrial biogenesis-related genes (Nrf1/NRF1, Tfam/TFAM) in HepG2 cells ( n = 6); (E, F) Protein expression and semi-quantification of key AdipoRs-AMPK pathway components in HepG2 cells ( n = 3); (G, H) Effects of FSP serum on inflammation, oxidative stress, mitochondrial function and lactate metabolism-related proteins with semi-quantitative analysis ( n = 3); (I) Immunofluorescence co-localization of SIRT1 and PGC-1α proteins in HepG2 cells (Scale bar: 20 μm, Total magnification: 200x); (J, K) Immunofluorescence detection of mitochondrial function proteins COXIV and TFAM (Scale bar: 20 μm, Total magnification: 200x); (L, M) Mitochondrial membrane potential assay (JC-1) and analysis (Scale bar: 20 μm, Total magnification: 200x, n = 5). Results are expressed as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to the vehicle group.

    Article Snippet: The specific antibodies used were as follows: AdipoR1 (Abclonal, A1509), AdipoR2 (Abclonal, A12777), p-AMPK (Abclonal, AP1441), AMPK (Abclonal, A1229), p-LKB1 (Abclonal, AP0601), LKB1 (Abclonal, A22636), PGC1α (Proteintech, 66369-1-Ig), SIRT1 (Proteintech, 13161-1-AP), LDHB (Proteintech, 14824-1-AP), COXIV (Cell Signaling Technology, 3E11), TFAM (Abclonal, A3173), NRF2 (Proteintech, 16396-1-AP), HO-1 (Abclonal, A19062), TNFα (Proteintech, 80258-6-RR), COX2 (Abclonal, A3560), and β-actin (Proteintech, 20536-1-AP).

    Techniques: Gene Expression, Expressing, Immunofluorescence, Membrane

    AMPK inhibition reverses biological effects of FSP serum intervention. (A) AMPK inhibition experiment in HepG2 cells under FSP serum intervention (Created in https://BioRender.com ); (B) Transcript levels of AMPK downstream effectors (SIRT1, PGC-1α) and mitochondrial genes (Nrf1/NRF1, Tfam/TFAM) ( n = 6); (C, D) Protein expression and semi-quantitative analysis of AMPK pathway molecules after AMPK inhibitor intervention ( n = 3); (E, F) Mitochondrial DNA copy number analysis ( n = 4); (G) Measurement of cellular ATP content ( n = 3); (H) Detection of cellular reactive oxygen species (ROS) levels ( n = 3); (I) SIRT1 and PGC-1α immunofluorescence co-localization (Scale bar: 20 μm, Total magnification: 200x); (J, K) Mitochondrial membrane potential (JC-1) staining and analysis (Scale bar: 20 μm, Total magnification: 200x, n = 5); (L–N) TEM analysis of mitochondrial ultrastructure with mitochondrial number ( n = 5) and area quantification ( n = 100) (Scale bar: 1/0.2 μm, Total magnification: 20,000/80,000x). Results are expressed as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001compared to the vehicle gro,Q22up.

    Journal: Frontiers in Nutrition

    Article Title: The herbal pair of Smilax glabra Roxb. and Ficus hirta Vahl. improves exercise performance by regulating mitochondrial function via the adiponection receptors-mediated AMPK signaling pathway

    doi: 10.3389/fnut.2026.1782001

    Figure Lengend Snippet: AMPK inhibition reverses biological effects of FSP serum intervention. (A) AMPK inhibition experiment in HepG2 cells under FSP serum intervention (Created in https://BioRender.com ); (B) Transcript levels of AMPK downstream effectors (SIRT1, PGC-1α) and mitochondrial genes (Nrf1/NRF1, Tfam/TFAM) ( n = 6); (C, D) Protein expression and semi-quantitative analysis of AMPK pathway molecules after AMPK inhibitor intervention ( n = 3); (E, F) Mitochondrial DNA copy number analysis ( n = 4); (G) Measurement of cellular ATP content ( n = 3); (H) Detection of cellular reactive oxygen species (ROS) levels ( n = 3); (I) SIRT1 and PGC-1α immunofluorescence co-localization (Scale bar: 20 μm, Total magnification: 200x); (J, K) Mitochondrial membrane potential (JC-1) staining and analysis (Scale bar: 20 μm, Total magnification: 200x, n = 5); (L–N) TEM analysis of mitochondrial ultrastructure with mitochondrial number ( n = 5) and area quantification ( n = 100) (Scale bar: 1/0.2 μm, Total magnification: 20,000/80,000x). Results are expressed as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001compared to the vehicle gro,Q22up.

    Article Snippet: The specific antibodies used were as follows: AdipoR1 (Abclonal, A1509), AdipoR2 (Abclonal, A12777), p-AMPK (Abclonal, AP1441), AMPK (Abclonal, A1229), p-LKB1 (Abclonal, AP0601), LKB1 (Abclonal, A22636), PGC1α (Proteintech, 66369-1-Ig), SIRT1 (Proteintech, 13161-1-AP), LDHB (Proteintech, 14824-1-AP), COXIV (Cell Signaling Technology, 3E11), TFAM (Abclonal, A3173), NRF2 (Proteintech, 16396-1-AP), HO-1 (Abclonal, A19062), TNFα (Proteintech, 80258-6-RR), COX2 (Abclonal, A3560), and β-actin (Proteintech, 20536-1-AP).

    Techniques: Inhibition, Expressing, Immunofluorescence, Membrane, Staining

    Synergistic activation of the AdipoRs-AMPK pathway by taxifolin and apigenin combination (A) Schematic design of monomer compound cell experiments (Created in https://BioRender.com ); (B) mRNA expression levels of STRT1, Ppargc1a, Nrf1 (NRF1), and Tfam (TFAM) ( n = 6); (C, D) Protein expression in AdipoRs-AMPK pathway and mitochondrial function ( n = 3); (E, F) Mitochondrial DNA copy number detection ( n = 6); (G) Intracellular ATP content measurement ( n = 3); (H) Detection of reactive oxygen species (ROS) levels ( n = 5); (I, J) Measurement of mitochondrial membrane potential (JC-1) (Scale bar: 20 μm, Total magnification: 200x, n = 5); (K–M) Transmission electron microscopy (TEM) observation of mitochondrial ultrastructure (Scale bar: 1/0.2 μm, Total magnification: 20,000/80,000x), statistical analysis of mitochondrial quantity ( n = 5) and mitochondrial area ( n = 100). Results are expressed as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to the vehicle group.

    Journal: Frontiers in Nutrition

    Article Title: The herbal pair of Smilax glabra Roxb. and Ficus hirta Vahl. improves exercise performance by regulating mitochondrial function via the adiponection receptors-mediated AMPK signaling pathway

    doi: 10.3389/fnut.2026.1782001

    Figure Lengend Snippet: Synergistic activation of the AdipoRs-AMPK pathway by taxifolin and apigenin combination (A) Schematic design of monomer compound cell experiments (Created in https://BioRender.com ); (B) mRNA expression levels of STRT1, Ppargc1a, Nrf1 (NRF1), and Tfam (TFAM) ( n = 6); (C, D) Protein expression in AdipoRs-AMPK pathway and mitochondrial function ( n = 3); (E, F) Mitochondrial DNA copy number detection ( n = 6); (G) Intracellular ATP content measurement ( n = 3); (H) Detection of reactive oxygen species (ROS) levels ( n = 5); (I, J) Measurement of mitochondrial membrane potential (JC-1) (Scale bar: 20 μm, Total magnification: 200x, n = 5); (K–M) Transmission electron microscopy (TEM) observation of mitochondrial ultrastructure (Scale bar: 1/0.2 μm, Total magnification: 20,000/80,000x), statistical analysis of mitochondrial quantity ( n = 5) and mitochondrial area ( n = 100). Results are expressed as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to the vehicle group.

    Article Snippet: The specific antibodies used were as follows: AdipoR1 (Abclonal, A1509), AdipoR2 (Abclonal, A12777), p-AMPK (Abclonal, AP1441), AMPK (Abclonal, A1229), p-LKB1 (Abclonal, AP0601), LKB1 (Abclonal, A22636), PGC1α (Proteintech, 66369-1-Ig), SIRT1 (Proteintech, 13161-1-AP), LDHB (Proteintech, 14824-1-AP), COXIV (Cell Signaling Technology, 3E11), TFAM (Abclonal, A3173), NRF2 (Proteintech, 16396-1-AP), HO-1 (Abclonal, A19062), TNFα (Proteintech, 80258-6-RR), COX2 (Abclonal, A3560), and β-actin (Proteintech, 20536-1-AP).

    Techniques: Activation Assay, Expressing, Membrane, Transmission Assay, Electron Microscopy

    Alteration of muscle FGF21 and downstream signaling in Ogg1 Tg mice. A , volcano plot of differentially expressed genes (DEGs) in skeletal muscle of Ogg1 Tg mice. Fgf21 is the most upregulated gene among all DEGs. B , Fgf21 gene expression was measured in major tissues of WT and Ogg1 Tg mice using quantitative PCR. Gene expressions were normalized to 18S rRNA expression. C , plasma FGF21was measured in WT and Ogg1 Tg mice using ELISA. D and E , phosphorylation of AMPK and ACC was measured in the skeletal muscle of WT and Ogg1 Tg mice using PAGE, and images were quantified using ImageJ. (n = 3–7); data are expressed as mean ± SD; ∗ p < 0.05 relative to WT. ACC, acetyl-CoA carboxylase; AMPK, AMP-activated protein; FGF21, fibroblast growth factor 21; Ogg1 Tg , OGG1-transgenic.

    Journal: The Journal of Biological Chemistry

    Article Title: OGG1 increases exercise endurance via elevated skeletal muscle FGF21

    doi: 10.1016/j.jbc.2026.111360

    Figure Lengend Snippet: Alteration of muscle FGF21 and downstream signaling in Ogg1 Tg mice. A , volcano plot of differentially expressed genes (DEGs) in skeletal muscle of Ogg1 Tg mice. Fgf21 is the most upregulated gene among all DEGs. B , Fgf21 gene expression was measured in major tissues of WT and Ogg1 Tg mice using quantitative PCR. Gene expressions were normalized to 18S rRNA expression. C , plasma FGF21was measured in WT and Ogg1 Tg mice using ELISA. D and E , phosphorylation of AMPK and ACC was measured in the skeletal muscle of WT and Ogg1 Tg mice using PAGE, and images were quantified using ImageJ. (n = 3–7); data are expressed as mean ± SD; ∗ p < 0.05 relative to WT. ACC, acetyl-CoA carboxylase; AMPK, AMP-activated protein; FGF21, fibroblast growth factor 21; Ogg1 Tg , OGG1-transgenic.

    Article Snippet: p-AMPK (Thr-172) , 2535- Cell Signaling Technologies , 1:1000.

    Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Phospho-proteomics, Transgenic Assay